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Dehalococcoides - which is present in uniquely high cell count number in our bioaugmentation agent - is the only known group of microorganisms capable of dechlorinating short-chain chlorinated aliphatic hydrocarbons (perchloroethene, trichloroethene, dichloroethenes, vinyl-chloride) to non-toxic ethene through reductive dechlorination under anaerobic conditions. The product contains 10E6 vynil-chloride reductase subunit A (vcrA) gene copies/ml.
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Taking into consideration the oxygen sensitivity of the organohalide-respiring microorganisms, bioaugmentation agent is dosed into properly anaerobic groundwater or soil with special dispensing containers. Upon request, our Team provides comprehensive professional provides professional support to remediation companies and specialists for the appropriate field application of the bioaugmentation agent.
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Aliphatic and aromatic hydrocarbons are typically present in contaminated soils in the form of total petroleum hydrocarbons (TPH), often in combination with carcinogenic polycyclic aromatic hydrocarbons (PAHs) (e.g. anthracene, phenanthrene, benzopyrene).
The product contains hydrocarbon-degrading microorganisms such as Lelliottia jeotgali, Pseudomonas mandelii, Rhodococcus qingshengii with a cell count values at least 10E9/ ml. |
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The microbial inoculum mainly used for the degradation of PAH – polycyclic aromatic hydrocarbons and the associated TPH – aliphatic hydrocarbon contaminants. The product contains hydrocarbon-degrading microorganisms such as Cupravidus necator, Pseudomonas benzenivorans, Pseudomonas extremaustralis with a cell count values at least 10E9/ml.
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The ability to degrade aliphatic, aromatic and polyaromatic hydrocarbons are widespread among the microorganisms. Various groups of bacteria and fungi are characterized with hydrocarbon-degradation potential, however, none of the microorganisms are capable to degrade the whole spectrum of this highly diverse group of compounds, therefore, diverse microbial communities characterized with different enzyme system are suitable for the complete degradation of complex contaminants.
The most effective solution to degrade complex pollutants is the application of microorganisms/microbial communities originated from contaminated sites. The application of predominately autochthonous microbial community adapted to certain pollution and environmental conditions (physico-chemical properties) has a greater chance of being effective, i.e. the use of site specific bioaugmentation agent. A major advantage of site-specific microbial inoculants is that while bioaugmentation products containing allochthonous microorganisms are not fully effective in all contaminated areas, microorganisms originating from and returned to a site can result in a significant reduction of contaminants. During bioremediation the application of a bioaugmentation agent containing effective microbial community adapted to on-site parameters is a half-success.
For the development of site-specific bioaugmentation agent, enrichment cultures and microcosms (ecosystem selection systems) were prepared using contaminated soils and groundwater to isolate and enrich microorganisms and microbial communities capable of hydrocarbon degradation. During the development and production process the concentration changes of the contaminant i.e. the biological degradation of the pollutant and the alteration of the microbial community, are monitored by gas chromatography (GC/GC-MS) and molecular biological methods (PCR, quantitative PCR, sequencing, microbial community analyses), respectively, in order to ensure excellent product quality. The developed site-specific bioaugmentation agent are produced in our fermentor park with more than 4500 litre capacity. Although, the development of site specific inocula are time- and energy-intensive, however, it provides an opportunity for effective remediation, complemented with biostimulation (e.g. application of carbon, nitrogen sources, electron donors, acceptors), the time required for remediation can be significantly reduced.
For the development of site-specific bioaugmentation agent, enrichment cultures and microcosms (ecosystem selection systems) were prepared using contaminated soils and groundwater to isolate and enrich microorganisms and microbial communities capable of hydrocarbon degradation. During the development and production process the concentration changes of the contaminant i.e. the biological degradation of the pollutant and the alteration of the microbial community, are monitored by gas chromatography (GC/GC-MS) and molecular biological methods (PCR, quantitative PCR, sequencing, microbial community analyses), respectively, in order to ensure excellent product quality. The developed site-specific bioaugmentation agent are produced in our fermentor park with more than 4500 litre capacity. Although, the development of site specific inocula are time- and energy-intensive, however, it provides an opportunity for effective remediation, complemented with biostimulation (e.g. application of carbon, nitrogen sources, electron donors, acceptors), the time required for remediation can be significantly reduced.
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